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. 2020 May 12;20:161. doi: 10.1186/s12935-020-01217-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — HOXD-AS1 binds to miR-326. a The potential binding sites were identified between HOXD-AS1 and miR-326 using bioinformatics analysis tool, starbase v2.0 (http://starbase.sysu.edu.cn/mirLncRNA.php). b Over-expression of HOXD-AS1 inhibited the levels of miR-326 in SMMC-7721 and HepG2 cells. c Cells were transfected with miR-NC or miR-326, and miR-326 transfection remarkably suppressed the levels of HOXD-AS1 in SMMC-7721 and HepG2 cells. d Luciferase reporter analysis suggested that HOXD-AS1 directly targeted miR-326. e The relationship between HOXD-AS1 and miR-326 in HCC tissues was evaluated. f The level of miR-326 was measured by qPCR method in 48 cases of HCC and adjacent non-cancerous tissues. ^**P<0.01 compared to normal. g Representative association between miR-326 and I-II or III-IV stage was shown. ^*P<0.05 compared to I-II. h Relative levels of miR-326 in patients with distant metastasis or without metastasis. ^*P<0.05 compared to no metastasis. i The level of miR-326 in four HCC cells and LO2 as detected by qPCR analysis. ^**P<0.01 compared to LO2 cell