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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Virology. 2019 Dec 6;541:52–62. doi: 10.1016/j.virol.2019.11.018

Fig. 3. Association of NS5-GFP with the centrosomes is maintained through mitosis.

Fig. 3.

HEK-293 cells were transiently transfected with NS5-GFP and nls-GFP expression plasmids.

A. NS5-GFP and nls-GFP expression were monitored by fluorescent microscopy (40X magnification).

B. Immunoblot analysis of NS5-GFP and nls-GFP expression. Blots were probed with anti-GFP antibodies. Molecular weight markers are shown at right (sizes in kDa). Predicted sizes of NS5-GFP and nls-GFP are 131 and 28 kDa, respectively.

C. Time-lapse images of an HEK-293 cell transiently expressing NS5-GFP undergoing mitosis.