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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Virology. 2019 Dec 6;541:52–62. doi: 10.1016/j.virol.2019.11.018

Fig. 4. NS5-GFP expression increases the time required to complete mitosis.

Fig. 4.

Expression of NS5-GFP and nls-GFP was induced by adding 2ug/mL tetracycline to the culture media.

A. Fluorescent and bright-field microscopic images of NS5-GFP and nls-GFP cell lines in the absence and presence of tetracycline.

B. Immunoblot analysis of lysates from NS5-GFP and nls-GFP cell lines in the presence of tetracycline. The blot was probed with anti-GFP and anti-actin antibodies. Molecular weight markers are shown at right (sizes in kDa).

C. Time-lapse images of a Flp-In™ T-REx™ 293 cell stably expressing NS5-GFP undergoing mitosis.

D. Flp-In™ T-REx™ 293 cells stably expressing ZIKV NS5-GFP or nls-GFP were analyzed for the time required to complete mitosis. A total of 160 cells from three independent experiments were analyzed for each cell line and the data were pooled. ** Unpaired t-test, p < 0.001.