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. 2020 May 11;17:154. doi: 10.1186/s12974-020-1731-x

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of BMSCs. Morphological observation of rat BMSCs (a) used in this study. Scale bar: 200 μm. b Flow cytometric analysis of BMSCs surface markers. The cells were positive for CD90, CD29, and CD105, and negative for CD45, CD34, and CD11b. c The multidifferentiation potential of BMSCs in vitro. Alizarin red S staining was used to evaluate osteogenic differentiation, Alcian blue staining was used to evaluate chondrogenic differentiation, and oil Red O staining was used to evaluate adipogenic differentiation. Scale bar: 30 μm