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Neutrophil chemotaxis induced by KCM-LA or KCM-GW is dependent on IL-8. (A, (B) The conditioned medium of HaCaT cells treated with 50 μM LA or 50 µM GW9508 was pre-treated for 10 min with anti-human IL-8 (1 μg/ml). The data are the mean ± SEM of 3 experiments. *P < 0.05, *** P <0.001 and ****P < 0.0001, using Tukey’s multiple comparison test. (C, D) Neutrophil chemotaxis was analyzed using 50 µM LA or 50 µM GW9508 as chemoattractants.
