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. 2020 May 5;10:196. doi: 10.3389/fcimb.2020.00196

Figure 2.

Figure 2

Histone H3 antibody attenuated pyroptosis induced by LPS in vitro. LPS (10 μg/ml) was used to stimulate the cells excluding control group. H3 antibody (10 μg/ml) was added into LPS + H3 antibody group 2h prior to LPS. The cells were harvested 24 h after the addition of LPS. (A) The mRNA expression (relative to GAPDH) of NLRP3, caspase-1, GSDMD, IL-1β, IL-18 in ANA-1 cells. (B–D) The protein levels of NLRP3, caspase-1, GSDMD, IL-1β, IL-18 in ANA-1 cells. Results were presented as mean ± SDs of three repetitions. *P < 0.05, compared with control group; #P < 0.05, compared with LPS group.