Fig. 3.

The effect of PGE₂ (10 μM) treatment of oocyte maturation medium on (a) glucose uptake and (b) lactate production by COCs, and on mRNA abundance of genes involved in glucose metabolism: cGLUT1, dGLUT4, eGFPT1, fGFPT2, gPFKP, and hLDHA in cumulus cells. Glucose uptake and lactate production are expressed as mean ± SEM of six independent experiments and presented as mg/dl per COC and mmol/L per COC, respectively. The values of mRNA expression are presented as arbitrary units and expressed as mean ± SEM of eight independent repeats (all cumulus cells separated from 10 respective oocytes in each replicate). Different letters indicate significant differences (P < 0.05), as determined by Student’s t-test