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. 2020 May 11;18:40. doi: 10.1186/s12958-020-00598-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effect of PGE₂ (10 μM) on mitochondrial distribution pattern in bovine oocytes after IVM. Panel (A) depicts representative fluorescent images of mitochondrial (a) homogeneous, (b) semi-peripheral, and (c) peripheral distribution in bovine oocytes after IVM. Bars = 25 μm. Panel (B) depicts quantitative analysis of PGE₂ effect on mitochondrial distribution pattern. The values are presented as percentage of COCs and expressed as mean ± SEM of three independent repeats (25 COCs per treatment group). Capital letters indicate statistical significance (P > 0.05) between two treatments whilst different small letters indicate significant differences (P < 0.05) within each treatment, as determined by two-way ANOVA followed by the Tukey multiple comparison test