Fig. 5.

The effect of PGE₂ (10 μM) on mitochondrial membrane potential (MMP) in bovine oocytes after IVM. Panel (A) depicts representative fluorescent images of oocytes with high (a-c) or low (d-f) polarization of mitochondria as detected by the JC-1 dye (a, d – green; b, e – red; c, f – merge fluorescence). Bars = 25 μm. Panel (B) depicts quantitative analysis of PGE₂ effect on mitochondrial membrane potential. The values are presented as the ratio of red fluorescence intensity to green fluorescence intensity and expressed as mean ± SEM of three independent repeats (25 COCs per treatment group). Different letters indicate significant differences (P < 0.05), as determined by Student’s t-test