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. 2020 May 11;18:40. doi: 10.1186/s12958-020-00598-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — The effect of PGE₂ (10 μM) treatment of oocyte maturation medium on apoptosis in COCs. Panel (A) depicts representative fluorescent images of bovine COCs used to TUNEL labeling: (a) negative control, (b) positive control, (c) control COCs, and (d) COCs matured in the presence of PGE₂. White arrows indicate TUNEL stained apoptotic nuclei (green) in contrast to DAPI stained nuclei (blue). Bars = 100 μm. Panel (B) depicts quantitative analysis of PGE₂ effect on apoptosis in COCs. The data are presented as a percentage of TUNEL positive apoptotic cells within all detected DAPI positive cells and expressed as mean ± SEM of three independent repeats (25 COCs per treatment group). Different letters indicate significant differences (P < 0.05), as determined by Student’s t-test