Fig. 5.

Tri-culture proteomic profile. Comparing the proteomic profile of the conditioned media from co- and tri-cultures after 48 h incubation with 5 μM LPS. a Heat map showing the relative cytokine concentrations scaled from 1 to 100. Proteins with a relative concentration of less than 10% of the maximum for all treatments were not included in the heat map but can be found in (Supplementary Figure 5). Hierarchical cluster analysis revealed three major groups of cytokines consisting of cytokines present in the conditioned media from all culture types (green), cytokines with increased expression in both control and LPS challenged tri-culture (orange), and cytokines with increased expression only in the LPS exposed tri-cultures (purple). b–j The relative concentrations of specific cytokines of interest. All graphs display mean ± SD (n = 3). The letters above the bars indicate statistically significant differences (p < 0.05, 2-way ANOVA with the Tukey test used to analyze the simple main effects if necessary). A full breakdown of the statistical analysis can be found in supplementary data table 4