Fig. 4.

PRLR-DbsAb activates T cells resulting in Cytokine release. Effector cells (huPBMCs) and target cells (T47D) were incubated with 100 ng/ml recombinant antibodies (PRLR mAb and PRLR-DbsAb) for 20 h (effect-to-target ratio of 10:1). The cells were stained by FACS antibody to analyze the expression of activation marker protein CD69 on the surface of PBMCs (a), CD4 (b) and CD8 (c) T cells. And the secreted levels of IFN- γ (d), TNF- α (e) and IL10 (f) in the culture supernatant were detected by Elisa method. The experiment was repeated three times and the data was expressed as the Mean ±SEM, ***P <0.001