Table 1.

The primers used in this study

Candida species	Primer name	Forward(5′-3′)	Reverse(5′-3′)	Amplified fragment size (bp)	References
C. albicans complexes	HWP1	GCTACCACTTCAGAATCATCATC	GCACCTTCAGTCGTAGAGACG	C. albicans:839 and 941 C. africana:700 C. dubliniensis:569	Shan,2014
C. glabrata complexes	GLA NIV BRA	CGGTTGGTGGGTGTTCTGC AGGGAGGAGTTTGTATCTTTCAAC GGGACGGTAAGTCTCCCG	ACCAGAGGGCGCAATGTG	C. glabrata:397 C. bracarensis: 223 C. nivariensis:293	Li,2014
C. parapsilosis complexes	mCPF mCOF mCMF	TTTGCTTTGGTAGGCCTTCTA TAAGTCAACTGATTAACTAAT AACTGCAATCCTTTTCTTTCTA	AATATCTGCAATTCATATTACT	C. parapsilosis:171 C. orthopsilosis:109 C. metapsilosis:217	Asadzadeh,2015
Rare yeast	NL1,NL4	GCATATCAATAAGCGGAGGAAAAG-3’	GGTCCGTGTTTCAAGACGG	500–600	Leaw,2006

¹Shan Y, Fan S, Liu X, et al. Prevalence of Candida albicans-closely related yeasts, Candida africana and Candida dubliniensis, in vulvovaginal candidiasis. Med Mycol, 2014, 52 (6): 636–40.

²Li J, Shan Y, Fan S, et al. Prevalence of Candida nivariensis and Candida bracarensis in vulvovaginal Candidiasis. Mycopathologia, 2014, 178 (3, 4): 279–83.

³Asadzadeh M, Ahmad S, Hagen F, et al. Simple, Low-Cost Detection of Candida parapsilosis complex isolates and molecular fingerprinting of Candida orthopsilosis strains in Kuwait by ITS region sequencing and amplified fragment length polymorphism analysis. PLoS One, 2015, 10 (11): e0142880.

⁴Leaw SN, Chang HC, Sun HF, et al. Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions. J Clin Microb, 2006, 44 (3): 693–9.