Fig. 5.

EGCG regulates LDLR expression and LDL uptake through PCSK9 in HepG2 cells. HepG2 cells were added rhPCSK9 or transfected with siRNA negative control (si-Control) or stealth siRNA for the knockdown of PCSK9 (si-PCSK9). a The levels of PCSK9 and LDLR were determined by western blot, GAPDH was used as the loading control. b Representative fluorescence microscopy images of cell‐associated Dil‐LDL (red), Hoechst‐stained nuclei (blue) and the overlay (upper). Fluorescence of isopropanol‐extracted Dil (520–570 nm, normalized to the cell protein) (lower). Data are presented as mean ± SEM (N = 3). **p < 0.01 compared with control. ##p < 0.01 compared with EGCG group. rhPCSK9: recombinant human