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. 2020 May 11;20:24. doi: 10.1186/s12896-020-00615-0

Table 2.

Plasmids used in this study

Name Key features Source
pZS4Int-tetR Gene encoding the TetR repressor under control of the constitutive PN25 promoter, SC101 ori, Spr [45]
pMA7CR_2.0 λ Red β-protein under control of the L-arabinose inducible PBAD, Cas9 under control of the anhydrotetracycline inducible PLtet, ColE1 ori, Ampr [30]
pMAZ-SK sgRNA targeting galK under control of the anhydrotetracycline inducible PLtet, L-rhamnose inducible plasmid self-killing mechanism, ColA ori, Kmr [30]
pMAZ-SK_x

pMAZ-SK with gRNA variants targeting x gene:

… dsbA

… dsbB

… skp

… sppA

… degP

 This study
pFREE Cas9 under control of the anhydrotetracycline inducible PLtet, gRNA targeting the most common replicons under control of the L-rhamnose inducible Prha, ColA ori, tetR, Kmr [31]
pSB-M1s pelB-scFv173–2-5-phoA fusion gene under control of the m-toluate inducible XylS/Pm promoter system, RK2 replicon, Kmr [25]
pGM29ompA gene encoding GM-CSF with ompA signal sequence under control of the m-toluate inducible XylS/Pm promoter system, RK2 replicon, Ampr [26]