Figure 5.

Representative reconstruction of a single astrocyte. Image of a 3D reconstruction of a representative z-stack showing Lck-GFP expressing astrocyte (green), neuronal and astrocyte nuclei stained with DAPI (blue) as well as IHC for GFAP (magenta) (I). The white signal represents colocalization of GFAP with Lck-GFP, clearly confirming the cell is indeed GFAP-positive astrocyte. Lck-GFP signal can be used to create a functional surface, capable of reporting volumetric features (II), which can be further used to completely isolate relevant signal and remove the background (III). Scale bar: 15 μm