Fig. 5.

Protective effect of morin against L. monocytogenes infection in vivo. a Mice were intraperitoneally injected with 1 × 10⁷ CFUs of L95 cells and treated with 100 mg/kg morin or DMSO 2 h after infection and at 8-h intervals. The survival rate of the infected mice was observed for 96 h. The mice were intraperitoneally injected with 2 × 10⁶ CFUs of L95 cells and treated with 100 mg/kg morin or DMSO 2 h after infection and again at 8-h intervals. Forty-eight hours after infection, all of the mice were euthanized, the tissues of the liver and spleen were homogenized, and the number of colonies in ground tissue was calculated viaserial dilution (e-f). The levels of IL-6, IL-1β and TNF-α were detected by ELISA (b-d). Ten mice were arranged to each group for survival assays and three independent trials were performed (30 in total). For the inflammation and burden of the bacteria assays three mice were arranged to each group and three independent trials were performed (9 in total).* indicates P < 0.05, and ** indicates P < 0.01. (Student’s t-test)