Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 30;11:697. doi: 10.3389/fmicb.2020.00697

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Liu, Wang, Zhang, Yang, Bai, Zheng, Zheng, Wang, Li and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Changes in the serine incorporator 5 (SERINC5)-induced non-glycosylation of hepatitis B virus (HBV) LHB, MHB, and SHB proteins were similar to the pattern of glycan mutants. (A) HEK293T cells were co-transfected with LHBs-flag, MHBs-flag, or SHBs-flag proteins or the indicated glycan mutants plus a negative control vector VR1012 or the SERINC5-HA. Immunoblot analysis of SERINC5 and LHB, MHB, SHB, expression using anti-HA or anti-flag antibodies. (B) SERINC5 had a similar effect on HBV LHB, MHB, and SHB proteins (C) but not BST-2 with tunicamycin. HEK293T cells were co-transfected as indicated. Twelve hours prior to cell harvest, the cells were treated with 10 μg/ml tunicamycin. Immunoblot analysis of SERINC5, LHB, MHB, SHB, and BST-2 expression. Tubulin served as a loading control.