FIGURE 6.

The interaction between serine incorporator 5 (SERINC5) and LHB, MHB, SHB proteins. (A) The localization of LHB proteins in HepG2 cells. LHB-ECFP or LHB-flag proteins were transfected into HepG2 cells. Cells were stained with anti-flag, anti-GM130, or anti-PDI antibody 48 h post-transfection. (B) The localization of SERINC5 in HepG2 cells. SERINC5-HA was transfected into HepG2 cells. Cells were stained with anti-HA plus anti-GM130 or anti-PDI antibody 48 h post-transfection. (C) SERINC5 co-localized with LHB proteins in the Golgi apparatus. HepG2 cells were co-transfected with LHB-ECFP and SERINC5-HA. Cells were stained with anti-HA plus anti-GM130 or anti-PDI antibody 48 h post-transfection. Images were taken with a Zeiss LZM710 confocal microscope. Scale bars: 10 μm. (D) LHB, MHB, and SHB proteins interacted with SERINC5 by a co-immunoprecipitation (co-IP) assay. HEK293T cells were co-transfected as indicated. At 48 h post-transfection, the cells were harvested and co-IP with anti-flag plus protein G beads. The cell lysates and co-IP products were analyzed by immunoblotting.