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. 2020 Jan 18;26(6):885–897. doi: 10.1093/ibd/izz330

FIGURE 2.

FIGURE 2.

Summarizes the effect of 1,25(OH)D2 on cathelicidin mRNA and protein levels by DLD1 colonic epithelial cells. A, In the absence of LPS, 1,25(OH)2D induced cathelicidin mRNA 3.46-fold (P < 0.01) and 3.04-fold (P < 0.01) at 0.01 nM and 0.1 nM, respectively, compared with controls. In the presence of LPS, 1,25(OH)2D induced cathelicidin mRNA 6.25-fold (P < 0.01), 3.46-fold (P < 0.01), 2.53-fold (P < 0.05), and 2.36-fold (P < 0.05) at 0.01 nM, 0.1 nM, 1 nM, and 10 nM, respectively, compared with controls. B, Treatment of 1,25(OH)D2 at lower concentrations (0 nM, 0.01 nM, 0.1 nM, 1 nM) with or without LPS did not increase cathelicidin protein concentrations measured from cell culture supernatants compared with controls. At 10 nM of 1,25(OH)D2 without LPS, cathelicidin protein concentration increased to 701.7 pg/mL (P < 0.01) vs 540.3 pg/mL from controls. At 10 nM of 1,25(OH)D2 with LPS, cathelicidin protein concentration increased to 735 pg/mL (P < 0.001) vs 530 pg/mL from controls. At 10 nM of 1,25(OH)D2, there was no difference in cathelicidin protein concentration between the LPS and non-LPS treatment groups. *P < 0.05, **P < 0.01, ***P < 0.001.

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