Table 1:
Information regarding the possible resolution for various de novo genome sequencing technologies
| Assembly resolution | Paired-end | Paired-end + long mate pair | Bionano | 10x Genomics |
|---|---|---|---|---|
| Gene content | Yes | Yes | No | Yes |
| Gene order | Yes | Yes | No | Yes |
| Repeat spanning | No | Yes | Yes | Yes |
| Structural variants | No | Yes | Yes | Yes |
| Haplotype resolution (phased genomes) | No | No | No | Yes |
When planning a genome assembly project, it is important to understand the strengths and limits of the various sequencing strategies available.