TABLE 1.

Assumptions required by employed methods.

Method	Assumptions
IVW	All instruments must be mutually independent, non-pleiotropic, and satisfy conditions (i-ii)
WME	All instruments must be mutually independent and satisfy conditions (i-ii). The no-pleiotropy condition must be satisfied by a subset of instruments that accounts for at least 50% of the total weight (minor weight condition).
MR-ER	All instruments must be mutually independent and satisfy conditions (i-ii). InSIDE must hold.

IVW, Inverse Variance Weighted; MR-ER, MR-Egger Regression; WME, Weighted Median Estimator.