Skip to main content
. 2020 May 5;11:592. doi: 10.3389/fphar.2020.00592

Figure 1.

Figure 1

Expression of Foxc1 was increased by intermittent PTH treatment and PTH1R silencing inhibited the expression of Foxc1 and PTH-induced osteogenic differentiation. (A) mRNA expression level of Foxc1 at different time points induced by intermittent PTH treatment for 7 days in rBMSCs. (B, C) Protein expression level and quantitative analysis of Foxc1 at different time points by intermittent PTH treatment for 7 days in rBMSCs. (D, E) PTH1R mRNA and protein expression at 48 h after transfection in rBMSCs in control group (si-Ctrl) and PTH1R silencing group (si-PTH1R). (F, G) Foxc1 mRNA and protein expression on the 3rd day in control group (Ctrl), intermittent PTH treatment group (PTH), PTH1R silencing group (si-PTH1R) and PTH1R silencing group with intermittent PTH treatment (si-PTH1R+PTH). (H, I) ALP staining and its quantitative analysis on the 7th day in control group (Ctrl), intermittent PTH treatment group (PTH), PTH1R silencing group (si-PTH1R) and PTH1R silencing group with intermittent PTH treatment (si-PTH1R+PTH). Scale bar = 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001.