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. 2020 May 5;11:592. doi: 10.3389/fphar.2020.00592

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Copyright © 2020 Ouyang, Li, Wang, Shen, Si and Shen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Foxc1 overexpression stimulated osteogenic differentiation induced by intermittent PTH treatment in vitro. (A) mRNA expression level of Foxc1 in Lv-vector and Lv-Foxc1 MC3T3-E1 cells at 48 h after transfection. (B, C) Protein expression level and quantitative analysis of Foxc1 at 48 h after transfection. (D) Cell proliferation rate in Lv-vector and Lv-Foxc1 MC3T3-E1 cells. (E) mRNA expression levels of the osteogenic markers, Runx2, Osx and Ocn on the 1st day, 4th day, and 7th day after exposed to intermittent PTH treatment. (F, G) Protein expression level and quantitative analysis of the osteogenic markers, Runx2, Osx, and Ocn on the 1st day, 4th day, and 7th day after exposed to intermittent PTH treatment. (H, J) ALP staining and activity in Lv-Foxc1 and Lv-vector MC3T3-E1 cells after 2 weeks of intermittent PTH treatment. (I, K) Alizarin red S staining and calcium concentration in Lv-Foxc1 and Lv-vector MC3T3-E1 cells after 3 weeks of intermittent PTH treatment. (I, K). Scale bar = 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001.