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. 2020 May 5;11:592. doi: 10.3389/fphar.2020.00592

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Copyright © 2020 Ouyang, Li, Wang, Shen, Si and Shen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Foxc1 stimulates osteogenic differentiation induced by intermittent PTH treatment in vivo. (A) Three-dimensional reconstruction images of the defect sites by micro-CT in different groups at 8 weeks. (B–E) BV percentage (B), trabecular number (C), trabecular thickness (D), and the number of trabecular (E) in different groups were quantified. (F–M) H&E staining of the defect sites in different groups at 8 weeks. (N) mRNA expression levels of Foxc1, Runx2, and Ocn in different groups at 8 weeks. Scale bar = 5 mm in (A), Scale bar = 10 μm in (F–I), Scale bar = 25 μm in (J–M). (J–M) are local amplification of boxed area in (F–I), respectively. Arrow indicates new bone formation. *p < 0.05, **p < 0.01, ***p < 0.001.