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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Cell Calcium. 2020 Feb 17;87:102182. doi: 10.1016/j.ceca.2020.102182

Figure 2. Immunoblot and caffeine-induced Ca2+ release measured in HEK293 cells expressing RyR1-WT or RyR1-ΔFF.

Figure 2.

(A) Immunoblots of HEK293 cells transfected with pCMV5-RyR1-WT or pCMV5-RyR1-ΔFF show 565 kDa WT and ΔFF bands. Both bands are absent in cells transfected with pCMV5 vector (not shown). (B) Ca2+ transients in HEK293 cells expressing WT-RyR1 or RyR1-ΔFF as changes of Fluo-4 fluorescence before and following the addition of 8 mM caffeine (arrow) to the bath solution. A.U., arbitrary units.