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. Author manuscript; available in PMC: 2021 May 1.

Published in final edited form as: Cell Calcium. 2020 Feb 17;87:102182. doi: 10.1016/j.ceca.2020.102182

Figure 3. — (A) Representative single-channel currents at −20 mV (upper and middle traces) or 0 mV (bottom traces) shown as downward deflections from the closed states (c−) in symmetrical 0.25 M KCl with 2 μM Ca²⁺ in the cis chamber (upper traces) and after the subsequent addition of EGTA to yield free Ca²⁺ of 0.1 μM (middle traces) or after the addition of 10 mM Ca²⁺ to the trans chamber (bottom traces). (B) Representative current-voltage relationships in 0.25 M symmetrical KCl (●) and after the addition of 10 mM trans Ca²⁺ (○). Current and time scales for single-channel traces are shown. Averaged P_o values and ion permeation properties are summarized in Table 1.

Figure 3. — (A) Representative single-channel currents at −20 mV (upper and middle traces) or 0 mV (bottom traces) shown as downward deflections from the closed states (c−) in symmetrical 0.25 M KCl with 2 μM Ca²⁺ in the cis chamber (upper traces) and after the subsequent addition of EGTA to yield free Ca²⁺ of 0.1 μM (middle traces) or after the addition of 10 mM Ca²⁺ to the trans chamber (bottom traces). (B) Representative current-voltage relationships in 0.25 M symmetrical KCl (●) and after the addition of 10 mM trans Ca²⁺ (○). Current and time scales for single-channel traces are shown. Averaged P_o values and ion permeation properties are summarized in Table 1.