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. 2020 Jan 21;59(9):3724–3731. doi: 10.1002/anie.201914799

Figure 4.

Figure 4

Simultaneous binding of L5, L18, and L25 with 5S rRNA. A) Schematic assembly of 5S rRNA with L5, L18, and L25 into RNP complex simultaneously. Structural data from PDB 4YBB. B) Binding chiral specificity between different chiral combinations of 5′‐FAM‐labeled 5S rRNA with L5, L18, and L25 simultaneously. C) Binding of 5S rRNA with L5, L18, and L25 in the presence of synthetic DNA probe complementary to the binding site of L5 (L5‐probe). D) Binding of 5S rRNA with L5, L18, and L25 in the presence of synthetic DNA probe complementary to the binding site of L18 (L18‐probe). E) Binding of 5S rRNA with L5, L18, and L25 in the presence of synthetic DNA probe complementary to the binding site of L25 (L25‐probe). All samples were incubated in TMK buffer at 33 °C for 45 min and analyzed by 8 % native PAGE.