Table 1.

Effects of compounds 1–5 on 10 μm Aβ42 fibril formation and oligomerization, as assessed by ThT‐ and BODIPY‐fluorescence spectroscopy, respectively. Compounds were tested at compound/Aβ42 ratios of 10:1 and 1:1 and compared with the values obtained for Aβ1–42 alone (t _1/2, F, and slope).

Fibril formation: Protocol A			Oligomerization: Protocol B
Compound	t 1/2 extension/	F reduction	Compound	t 1/2 extension/	F reduction	Slope
(compound/Aβ ratio)	reduction[a]	[%][b]	(compound/Aβ ratio)	reduction[a]	[%][b]	reduction [%][c]
1 (10:1)	n.a.[c]	−85±2	1 (10:1)	n.a.	−75	−99
1 (1:1)	n.a.	−89±2	1 (1:1)	n.a.	−73	−95
2 (10:1)	n.e.[d]	n.e.	2 (10:1)	n.a.[d]	−81	−94
			2 (5:1)	n.a.[d]	−82	−100
2 (1:1)	n.e.	n.e.	2 (1:1)	r.a.[e] (from 110 min)	−80	−9 (110 min)
3 (10:1)	+77±15	n.e.	3 (10:1)	n.a.	−78	−91
3 (1:1)	+39±36	n.e.	3 (1:1)	r.a. (from 120 min)	−47	+8
4 (10:1)	−16±8	−14±4	4 (10:1)	n.a.	−78	−95
4 (1:1)	−15±10	−15±5	4 (1:1)	n.e.	−8	−15
5 (10:1)	n.a.	−85±2	5 (10:1)	n.a.	−86	−93
5 (1:1)	+154±23	n.e.	5 (1:1)	n.a.	−84	−94

See the Supporting Information for details of the calculation of [a] t _1/2 extension, [b] F reduction, and [c] slope reduction. [d] n.a.: no aggregation. [e] r.a.: reduction of aggregation. [f] n.e.: no effect. Parameters are calculated from the mean curves, as derived by statistical analysis of data after triplicate measurements for each condition and at least two independent experiments.