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. Author manuscript; available in PMC: 2020 May 12.
Published in final edited form as: ACS Chem Neurosci. 2019 Apr 29;10(7):3143–3153. doi: 10.1021/acschemneuro.8b00596

Figure 6. Transduction of N2A-1B cells with a lentiviral CRISPR/Cas9 vector induces specific and efficient knockout (KO) of β-arrestin 1 and β-arrestin 2.

Figure 6.

(a) Lentiviral CRISPR/Cas9 vectors were used to generate stable β-arrestin KO N2A-1B cell lines. (b) Transduction with a non-targeting control vector does not alter the expression of β-arrestin 1 or β-arrestin 2. Transduction with CRISPR/Cas9 vectors specific for β-arrestin 1 does not alter the expression of β-arrestin 2, and transduction with vectors specific for β-arrestin 2 does not alter the expression of β-arrestin 1.