Table 2.
Immunoreactivity analysis of tissue inhibitor of metalloproteinase 1 (TIMP1), TIMP2, TIMP3 and TIMP4
| (Dysmorphic) Neurons | Glia | Giant cells | ||||
|---|---|---|---|---|---|---|
| OD | Rel. no. | OD | Rel. no. | OD | Rel. no. | |
| TIMP1 | ||||||
| Control | 4 ± 6 | 2 (1–3) | 27 ± 6 | 1.5 (1–2) | – | – |
| Tuber | 25 ± 5 | 2.5 (1–3) | 36 ± 5^^ | 2.5 (2–3)* | 14 ± 3 | 2.5 (1–3) |
| Perituber | 0 ± 4 | 3 (2–3) | 3 ± 5* | 1 (1–3) | – | – |
| TIMP2 | ||||||
| Control | 4 ± 6 | 3 (2–3) | 27 ± 6 | 1 | – | – |
| Tuber | 33 ± 4 | 3 | 39 ± 5 | 3*** , ^^ | 29 ± 4 | 3 |
| Perituber | 21 ± 2 | 3 | 24 ± 4 | 2** | – | – |
| TIMP3 | ||||||
| Control | 24 ± 2 | 2.5 (2–3) | 8 ± 1 | 1 | – | – |
| Tuber | 93 ± 11 | 3 | 58 ± 12** | 2.5 (2–3)** | 48 ± 11 | 3 |
| Perituber | 44 ± 6 | 3 | 32 ± 9* | 2.5 (2–3)** | – | – |
| TIMP4 | ||||||
| Control | 1 ± 4 | 3 (2–3) | 64 ± 7 | 1 (1–2) | – | – |
| Tuber | 50 ± 13 | 3 (2–3) | 64 ± 6 | 3 (2–3)** , ^ | 38 ± 8 | 3 (2–3) |
| Perituber | 10 ± 5 | 3 | 53 ± 8 | 2 (2–3)** | – | – |
The background‐corrected optical density (OD) is given as mean ± SE of the mean. The relative number (Rel. no.) of positive cells is given as median (minimum–maximum) and is defined as follows: 0: no; 1: single to 10%; 2: 11–50%; 3: >50%. Different compared to control, *P < 0.05, **P < 0.01, ***P < 0.001. Different compared to tuberous sclerosis complex perituber, ^ P < 0.05, ^^ P < 0.01.