A. EnzS1-C4-2 cells were pre-treated with Enz for three days, and serum-starved overnight in media containing 0.1% BSA, then stimulated with 50ng/ml human EGF for the indicated time periods. P-AKT-S473, p-AKT-T308 and p-ERK1/2 (T202/Y204) levels were detected by western blot. B-D. Quantification results of p-ERK 1/2, p-AKT-S473, and p-AKT-T308. The data shown represent the mean ± SEM of three independent experiments. *p < 0.05, ***p < 0.001.