Extended Data Fig. 6 |. Nuclear function as a mechanical guide.

a, DC expressing lamin(D50)–GFP (lamin(D50) is also known as progerin) migrating through a four-way pore-size decision (4 experiments). b Quantification of pore-size preference in a, divided into GFP⁺ and GFP⁻ regions (4 experiments, 293 cells; unpaired t-test; *P = 0.0118). c Pore-size preference of 3T3 fibroblasts expressing lamin(D50)–GFP (or wild-type lamin–GFP as control) migrating in pillar arrays with differently sized pores (3 experiments, 606 pore passages; two-way ANOVA with Tukey’s test; ****P < 0.0001). d, Low hydrostatic pushing of cells and cellular fragments through four-way pore-size decision (n = 66). e, DC (nucleus labelled, Hoechst, blue) loaded with a 6-μm polystyrene bead (cyan) migrating through a pillar array with 5 μm and 8 μm pores (3 experiments). f, Graph showing where the intracellular bead gets stuck (3 experiments, 29 cells; one-way ANOVA with Tukey’s test; ****P < 0.0001, **P = 0.0016 (8 μm vs between pillar rows), **P = 0.0035 (5 μm vs between pillar rows)). g, Graph showing intracellular location of the bead when it gets stuck (3 experiments, 29 cells; unpaired t-test; **P = 0.0039). h, Frequency of cellular reorientation of DCs with an intracellular bead after getting stuck (3 experiments, 29 cells; unpaired t-test; **P = 0.0011). Data are mean ± s.d.