FIGURE 2.

Characterization of E8_I-CD4 transgenic mice. A, Single-cell suspensions of thymocytes from littermate (WT) and E8_I-CD4 mice were stained with fluorochrome-conjugated Abs as indicated and analyzed by flow cytometry. Left, TCRβ expression and the frequency of TCRβ^high thymocytes; right, average number ±SEM) of thymocyte subsets from WT (n = 6) and E8_I-CD4 (n = 6). CD4 lineage and CD8 lineage thymocytes were defined as CD24^lowCD4⁺ and CD24^lowCD8⁺, respectively. B, Thymocytes and LN cells from WT and E8_I-CD4 transgenic mice were analyzed for CD4, CD8, and CD24 expression. Mature CD8 lineage cells in thymus and LN from WT mice are shown in blue, whereas mature CD8 lineage cells from E8_I-CD4 mice are shown in red. Numbers in parentheses indicate total numbers of cells, while numbers in boxes indicate the frequency (percent) of cells in that box. Data are displayed on a 4-decade log scale and are representative of five independent experiments. C, Comparison of CD8 lineage T cells in WT and E8_I-CD4 transgenic mice. Surface expression of the indicated proteins on CD8 lineage thymocytes and LN T cells from WT mice (blue line) and E8_I-CD4 transgenic mice (red line) are shown. Data are displayed on a 4-decade log scale and are representative of five independent experiments.