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. 2020 May 8;12:3223–3235. doi: 10.2147/CMAR.S237582

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© 2020 Dai et al.

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WDR5 up-regulates H3K4me3 expression and increases H3K4me3 binding to the promoter region of ZEB1. (A) The expression of H3K4me3 was assayed by Western blot in SW 1783-shWDR5 and its control cells. (B) The expression of H3K4me3 was assayed by Western blot in U 343-WDR5 and its control cells. (C) Schematic presentation of three regions relative to the ZEB1transcriptional start site used as primers to test H3K4me3 occupied abundance. (D) ChIP-qPCR was performed to assess H3K4me3 occupancy to ZEB1 transcriptional start site in SW 1783-shWDR5 and its control cells. IgG was used as negative control. (E) ChIP-qPCR was performed to assess H3K4me3 occupancy to ZEB1 transcriptional start site in U 343-WDR5 and its control cells. IgG was used as negative control. All experiments were repeated at least three times, and representative data are shown. **P<0.01 is based on Student’s t-test. Error bars indicate standard deviation.

Abbreviations: H3K4me3, Trimethylation of lysine 4 on histone H3 protein subunit; ZEB1, Zinc finger E-box binding homeobox 1; WDR5, WD repeat domain 5.