Table 1.

Summary of the Clinical Parameters, Immunohistochemistry and Molecular Findings of 15 E-GBM Patients

Case	Gender	Age	Location	Symptoms	Tumor Size	Necrosis	EZH2	Ki-67	MGMT-MSP	BRAFV600E (HRM-PCR)	IDH1 (HRM-PCR)	1p/19q(FISH)	Treatment	Clinical Outcomes	Interval to Demise or Survival
1	Male	45	Left temporal -occipital	A history of anaplastic astrocytoma for 5 years	4×3.5×2cm	Extensive	++	10%	Unmethylation	Mutation	WT	No deletion	Surgery	Died	OS: 5 months
2	Male	32	Left temporal	Headache for 1 month	5.3×4.3 cm	Extensive	++	25%	Unmethylation	WT	WT	No deletion	Surgery	Died	OS: 12 months
3	Female	32	Right frontal	Headache and sudden disturbance of consciousness	4.3×4.1 cm	Extensive	++	20%	Unmethylation	Mutation	WT	No deletion	Surgery+ chemotherapy (Nimustine)	Recurrence 3 months after surgery, died	OS: 8 months
4	Male	52	Right temporal	Dizziness and headache for 10 hours	3×2×1.5cm	Extensive	++	10%	Unmethylation	WT	WT	No deletion	Surgery	Died	OS: 3 months
5	Female	18	Left thalamus	Nausea and vomiting for 3 weeks, limb weakness and palpitation for 2 days	2.8×2.8×3.2 cm	Extensive	+	10%	Unmethylation	WT	WT	No deletion	Surgery	Died	OS: one week
6	Male	40	Left occipital	Dizziness and headache for 8 months, aggravation and vomiting for 1 month	5×4×4 cm	Focal	+	30%	Methylation	Mutation	WT	No deletion	Surgery + radiotherapy	Recurrence 2 months after surgery,died	OS: 15 months
7	Male	44	Right frontal	Dizziness, headache and vomiting for 4 days	3×2×2 cm	Focal	+	15%	Methylation	Mutation	WT	No deletion	Surgery + chemotherapy (Nimustine)+radiotherapy	Recurrence three times after surgery,died	OS: 32months
8	Male	20	Right temporal	Dizziness and headache for 3 months, aggravation and vomiting for 1 month	3.1×3.2 cm	Limited	-	25%	Unmethylation	Mutation	WT	No deletion	Surgery	Died	OS: 15 months
9	Male	34	Right frontal	Dizziness and headache for one year accompanied by double vision of left eye for half a month	6.5×5.0×4.3cm	Extensive	-	30%	Unmethylation	WT	WT	No deletion	Surgery	Died	OS: 2 weeks
10	Male	77	Left temporal	Weakness of left limb for 1 month	5.5×5.2×3.5cm	Extensive	++	40%	Methylation	WT	WT	No deletion	Surgery	Died	OS: 2 weeks
11	Male	29	Right frontal	Dizziness for 4 days, aggravation and vomiting for 2 days	9.2×9.0×2.2cm	Extensive	++	20%	Methylation	mutation	WT	No deletion	Surgery + chemotherapy (TMZ) + radiotherapy	Died	OS:7 months
12	Male	58	Left frontal and temporal lobe	Dizziness for one year	4.8×4.2×3.8cm	Extensive	++	30%	Methylation	WT	WT	No deletion	Surgery	Alive	3 months
13	Male	25	Right temporal and occipital lobe	Headache for one month	4.0×4.0×1.0cm	Extensive	+	10%	Methylation	Mutation	WT	No deletion	Surgery + chemotherapy (TMZ)	Alive	2 months
14	Female	32	Right frontal and temporal lobe	Headache for half a month	5.0×5.0×4.0cm	Extensive	++	30%	Unmethylation	WT	WT	No deletion	Surgery	Alive	One month
15	Male	56	Right frontal and parietal lobe	Headache for 3 months and abnormal speech for 2 weeks	3.5×3.0×1.5cm	Extensive	++	20%	Methylation	WT	WT	No deletion	Surgery	Died	OS:9 months

Abbreviations: E-GBM, epithelioid glioblastoma; OS, overall survival; WT, wild type; EZH2, enhancer of zeste 2, EZH2 staining was graded as “−”, “+”, and “++”, when the percent positive nuclei were <5%, 5–25%, and >25%, respectively; MGMT, O-6-methylguanine-DNA methyltransferase; MSP, methylation-specific polymerase chain reaction; HRM-PCR, high-resolution melt polymerase chain reaction; BRAF, v-raf murine sarcoma viral oncogene homolog B1; IDH1, isocitrate dehydrogenase1; FISH, fluorescence in situ hybridization; Ki-67, antigen Ki67; TMZ, temozolomide.