Figure 3.

Body mass (A), liver (B), gastrocnemius muscle (C) and gonadal white adipose tissue (gWAT) (D) tissue masses, and gWAT adipocyte area (E) and angiogenic sprouting (F) in gWAT explants from normal chow (NC) and high fat diet (HF) fed wildtype (WT) and roundabout receptor 4 (Robo4) knockout (KO) mice. WT and KO mice were fed either NC or HF (40% fat by kcal) for 8–10 weeks before euthanasia for tissue collection. Adipocyte area was assessed in hematoxylin and eosin stained sections of paraffin embedded gWAT and are presented as the mean area of at least 200 adipocytes/animal. Angiogenic sprouting was assessed in gWAT explants (~1mm³) embedded in matrigel and treated with vascular endothelial growth factor (0.5 mg/ml). Six explants were used per animal and data presented is the mean number of sprouts in these 6 cultures, 5 days after embedding. *denotes difference from NC fed WT, †denotes difference from HF fed of same genotype, Data are means ± SEM, p≤0.05