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. Author manuscript; available in PMC: 2020 May 12.
Published in final edited form as: Glia. 2019 Aug 24;67(11):2178–2202. doi: 10.1002/glia.23702

FIGURE 2.

FIGURE 2

Microinjections of a protease-activated receptor-1 (PAR1) agonist into the intact spinal white matter induces cell proliferation including OPCs within 24 hr. (a) Injection site of PAR agonist (TFLLR-NH2, 60 nmol/1.5 μL, Abgent, San Diego, California) immunolabeled for NG2 (for OPCs; black) and Ki67 (brown) to label dividing cells. Two double-labeled NG2 cells are indicated by arrowheads. Vehicle (phosphate-buffered saline, PBS) injection sites contained no Ki67+ cells. (b) The number of Ki67+ cells expressing NG2 were quantified in the injection site. PAR1 agonist injection significantly increased the number in dividing NG2 cells. (c) The total number of Ki67+ cells was also quantified in the injection sites revealing a significant increase in overall proliferation in PAR agonist-injected tissue compared to PBS control. ***p < .001 t-tailed t test