Skip to main content
. 2020 Apr 14;19(10):1122–1131. doi: 10.1080/15384101.2020.1748949

Figure 4.

Figure 4.

PCAT1 promotes proliferation, migration and invasion of Caki2 and ACHN cells by upregulating YAP via sponging miR-656 and miR-539. (a) Bioinformatics analysis shows the miR-656 and miR-539 binding sites on YAP 3ʹUTR. (b) Dual-luciferase report assay showed that miR-656 and miR-539 reduced the luciferase activity of PCAT1-WT, but not of PCAT1-MUT. (c) Knockdown PCAT1 with si-PCAT1#1 suppressed the mRNA and protein levels of YAP, while its inhibition was dramatically alleviated by cotransfection of miR-656 or miR-539 inhibitor. (d) The expression levels of YAP in ccRCC tumors and adjacent normal tissues. (e) Spearman’s correlation analysis revealed that the expression levels of PCAT1 and YAP exhibited a positive correlation (p < 0.0001). (f–h) Cotransfection with miR-656 or miR-539 inhibitor or YAP plasmid into Caki-2 and ACHN could alleviate the inhibitory effects of PCAT1 on cell proliferation, migration and invasion of Caki-2 and ACHN. *p < 0.05, **p < 0.01 versus si-NC group.