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. 2020 Apr 19;19(10):1186–1199. doi: 10.1080/15384101.2020.1749466

Figure 2.

Figure 2.

Deficiency of SNHG22 expression inhibits the proliferation, migration, and invasion but promotes HTH83 and TPC-1 cell apoptosis.

(a) RT–qPCR analysis of SNHG22 expression in HTH83 and TPC-1 cells after transfection with si-SNHG22 or si-NC. RT-qPCR analysis was repeated at least thrice. *P < 0.05 vs. si-NC. (b) The growth curves of si-SNHG22- or si-NC-transfected HTH83 and TPC-1 cells were determined via a CCK-8 assay. CCK-8 assay was repeated at least three times. *P < 0.05 vs. si-NC. (c) Flow cytometry analysis was applied to estimate the proportion of apoptotic HTH83 and TPC-1 cells after SNHG22 knockdown. Flow cytometry analysis was repeated at least three times. *P < 0.05 vs. si-NC. (d, e) The migratory and invasive capacities of SNHG22 silencing-HTH83 and TPC-1 cells were tested via in vitro cell migration and invasion assays. The results were expressed as the number of migrated and invaded cells per field, respectively. In vitro cell migration and invasion assays were repeated at least thrice. *P < 0.05 vs. si-NC.