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. 2020 Apr 19;19(10):1186–1199. doi: 10.1080/15384101.2020.1749466

Figure 4.

Figure 4.

SNHG22 positively regulates ZEB1 expression in PTC cells.

(a, b) ZEB1 mRNA and protein levels in HTH83 and TPC-1 cells after SNHG22 silencing were measured using RT–qPCR and western blotting, respectively. RT–qPCR and western blotting were repeated at least thrice. *P < 0.05 vs. si-NC. (c) RT–qPCR analysis quantified the efficiency of miR-429 inhibitor transfection in HTH83 and TPC-1 cells. RT–qPCR was repeated at least thrice. *P < 0.05 vs. NC inhibitor. (d, e) SNHG22-deficient HTH83 and TPC-1 cells were further transfected with miR-429 inhibitor or NC inhibitor. After transfection, RT–qPCR and western blotting revealed ZEB1 mRNA and protein expression in aforementioned cells. RT–qPCR and western blotting were repeated at least thrice. *P < 0.05 vs. si-NC. #P < 0.05 vs. si-SNHG22+ NC inhibitor. (f) ZEB1 mRNA expression in 65 pairs of PTC tissues and paired adjacent non-tumor tissues was measured using RT–qPCR. RT–qPCR was repeated at least thrice. *P < 0.05 vs. paired adjacent non-tumor tissues. (g) The interaction between SNHG22 and ZEB1 mRNA in PTC tissues was determined using Spearman’s correlation analysis. r = 0.5695, P < 0.0001.