Figure 4.

LncRNA CRNDE affects the proliferation and apoptosis of VSMCs by regulating Smad3 through Bcl-3. pcDNA, pcDNA-CRNDE or pcDNA-CRNDE+si-Bcl-3 was transfected into mouse VSMCs and stimulated with AngII. (a) Western blot was used to detect the protein levels of Bcl-3 and Smad3. (B–D) MTT and EdU assays were used to detect the proliferation of VSMCs and the percentage of EdU positive cells, respectively. (E,F) Flow cytometry was performed to analyze the apoptosis of VSMCs. *P < 0.05 (compared to AngII+pcDNA group), ^#P < 0.05 (compared to AngII+pcDNA-CRNDE group).