Figure 1.

miR-206 expression was downregulated in liver CSCs.

(a). Real-time PCR was performed to examine the expression of miR-206 in hepatoma spheroids. (n = 3). (b). miR-206 expression in serial passages of Huh7 and HCCLM3 spheroids was analyzed by real-time PCR. (n = 3). (c). HCCLM3 and Huh7 cell–derived spheroids were trypsinized and cultured in attachment conditions. miR-206 expression in spheroids versus reattached cells was compared by real-time PCR. (n = 3). (d). Expression of miR-206 in CD133⁺ or EpCAM⁺ subpopulation of Huh7 cells was examined by real-time PCR. (n = 3). (e). Expression of miR-206 in CD133⁺ or EpCAM⁺ subpopulation of HCCLM3 cells was examined by real-time PCR. (n = 3). (f). Real-time PCR was performed to check the expression of miR-206 in cisplatin-resistant HCC xenograft. (n = 3). (g). Expression of miR-206 in non-recurrence and recurrence HCC patients was examined by real-time PCR. (n = 3)(Data are represented as mean±s.d.; *P < 0.05; two-tailed Student’s t-test.)