Figure 2.

Interference of miR-552 suppresses laryngocarcinoma cells' proliferation.

(a) The interference effect of miR-552 in M4E and Hep-2 cells was checked by real-time PCR assay. (b) Cell proliferation was measured using CCK-8 assays in M4E miR-552 sponge or Hep-2 miR-552 sponge and their control cells. (c) Colony formation assays of M4E miR-552 sponge or Hep-2 miR-552 sponge and their control cells. (d) Cell proliferation was assessed using EdU immunofluorescence staining (red) in M4E miR-552 sponge or Hep-2 miR-552 sponge and their control cells. Nuclei were stained with Hoechst 33342 (blue). (e) Hep-2 miR-552 sponge and its control cells were implanted subcutaneously to induce xenograft tumor in nude mice. Mice were sacrificed at fourth weeks after inoculation; tumor was excised, pictured and weighed.