Figure 6.

Inhibition of exosomal miR-26b-5p partially reverses exosome functions in microglia M1 polarization. (a), Immunofluorescence staining of M1 phenotype microglia marker iNOS and M2 phenotype microglia marker Arg 1; (b), CD38⁺CD206⁻ was categorized as M1 phenotype microglia while CD38⁻CD206⁺ was M2 phenotype microglia detected by flow cytometry; (c), ELISA was performed to determine pro-inflammation factors TNF-ɑ, IL-6, and CCL-2 protein contents. Then, OGD/R- and exosomes- or PBS-treated microglia was co-cultured with SH-SY5Y or PC12 cells. D-E, EdU staining (d) and Hoechst 33258 staining (e) were performed to determine SH-SY5Y and PC12 cells viability and apoptosis. Data are expressed as mean ± standard deviation and analyzed with the unpaired t test (data in panels a and b) or one-way ANOVA (data in panels c and d), followed by Tukey’s multiple comparisons tests. *p < 0.05. Three independent experiments were performed.