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. 2020 Apr 8;19(10):1222–1235. doi: 10.1080/15384101.2020.1749468

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Figure 6. — Restoration of the Bcl-2 level abrogates the impact of RUSC1-AS1 knockdown on the malignant phenotype of cervical cancer cells. (a) Bcl-2 protein expression was confirmed in the pCMV-Bcl-2–transfected or pCMV-transfected HeLa and SiHa cells by western blotting. (b–e) Si-RUSC1-AS1 was cotransfected with either pCMV-Bcl-2 or pCMV into HeLa and SiHa cells. Measurement of proliferation, apoptosis, migration, and invasiveness of the above-mentioned cells was performed by the CCK-8 assay, flow-cytometric analysis, and migration and invasion assays, respectively. *P < 0.05 and **P < 0.01.