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. 2020 Apr 3;31(5):1009–1023. doi: 10.1681/ASN.2019080790

Table 2.

Sequences of primers used for real-time PCR

Name Forward Reverse
P0 5′-AAT​CTC​CAG​AGG​CAC​CAT​TG-3′ 5′-GTT​CAG​CAT​GTT​CAG​CAG​TG-3′
α-ENaC 5′-CAG​ACT​TGG​AGC​TTT​GAC​AAG​GA-3′ 5′-ACT​TCT​CTG​TGC​CTT​GTT​TAT​ATG​TGT​T-3′
β-ENaC 5′-CAG​ACT​GGG​CCT​ATG​CTA​TCT​AAA-3′ 5′-ACA​TGC​TGA​GGC​AGG​TCT​CTC​T-3′
γ-ENaC 5′-CCG​AGA​TCG​AGA​CAG​CAA​TGT-3′ 5′-CGCTCA GCTTGA AGGATTCTG-3′
Claudin-8 5′-GTG​CTG​CGT​CCG​TCT​TGG​CT-3′ 5′-TCG​TCC​CCC​GTG​CAT​CTG​GT-3′
Claudin-4 5′-AAGTGCACCAACTGC ATGGA-3′ 5′-GGC​TCC​GGC​GGT​GAT​C-3′
Claudin-7 5′-AAG CGA AGA AGG CCC GAA TA-3′ 5′-GCA AGA CCT GCC ACA ATG AA-3′
ZO-1 5′-TTA​TGC​GCA​GTG​GTA​TCC​AAT​T-3′ 5′-TCC​GGA​CAC​AAC​CTC​ATC​CT-3′