Figure 2.

Ccl2 expression by tubular cells is required for STAT3 activation in response to Pkd1 disruption. (A) Western blot of STAT3 phosphorylation in kidney lysates from 13-week-old control, iPkd1^ΔTub, and iPkd1^ΔTub; iCcl2^ΔTub mice (7 weeks after the completion of doxycycline treatment). Each lane indicates one individual mouse. (B and C) Quantification of (B) Socs3 and (C) Lcn2 mRNA abundance in kidneys from 13-week-old control, iPkd1^ΔTub, and iPkd1^ΔTub; iCcl2^ΔTub mice. (D) Representative images and (E and F) quantification of pSTAT3^Y705 immunostaining in kidneys from 13-week-old control, iPkd1^ΔTub, and iPkd1^ΔTub; iCcl2^ΔTub mice. Scale bar, 50 µm. (B, C, E, and F) Each dot represents one individual mouse. One-way ANOVA followed by Tukey–Kramer test, *P<0.05, **P<0.01, ***P<0.001. AU, arbitrary units.