Figure 1.

Cold-storage time determines tubular cell injury and proliferation in transplanted kidneys. (A) Diagram showing the experimental design. The left kidney was collected from C57BL/6 donor mice for 0.5–14 hours of cold storage, followed by transplantation into syngeneic recipient mice for 24 hours of reperfusion. The right kidney of donor mice without cold-storage transplantation was used as sham control. (B) Representative images of hematoxylin and eosin (H&E) staining, TUNEL assay, and Ki67 immunohistochemistry. Scale bars, 0.2 mm. (C) Tubular damage score. (D) Quantification of TUNEL-positive cells. (E) Quantification of Ki67-positive tubular or interstitial cells. (F) Immunoblot analysis of PCNA. Cyclophilin B (CycB) was used as the loading control. Data in (B–D) are expressed as mean±SD (n=4–6). *P<0.05 versus sham control. C, cold; Rep, reperfusion.