Fig 3. MicroRNAs miR-96 and miR-183 regulate cell viability.
50nM of mirVana™ miRNA mimics for miR-96, miR-183, or Negative Control #1 were transfected into MCF-7M cells (A, B) and 50nM of mirVana™ miRNA inhibitors for miR-96, miR-183, or Negative Control #1 were transfected into MCF-7 cells (C, D). Cell counts were determined by staining with 0.4% trypan blue solution after 24 hours (A, C) or 48 hours (B, D), and averages and standard deviations of triplicate counts over three independent experiments are reported. (E) Calculated average percent change in viable cells for mock treatment vs. miRNA mimic or inhibitor treatment 48 hours post-transfection ± standard deviation. (F) A MTT Assay was performed to obtain an additional quantitative measure of cell viability in MCF-7M cells. Statistical significance was determined using a one-way ANOVA followed by a Tukey-Kramer post-hoc test (*p < .05; **p < .01).